Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human AMPK1/AMPK2 around the phosphorylation site of Serine 485 or Serine 491.
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
Q13131 P54646
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100
ELISA: 1:10000
Specificity Sensitivity:
AMPK1/AMPK2(Ab-485/491) Antibody detects endogenous levels of total AMPK1/AMPK2 protein.
References:
Kim JE, et al. (2005) J Proteome Res. 4(4): 1339-1346.
Woods A, et al. (2003) J Biol Chem. 278(31): 28434-28442.
Immunohistochemical analysis of paraffin-embedded colon carcinoma, using AMPK1/AMPK2(Ab-485/491) Antibody (#2B7007). Left: Untreated; Right: Treated with synthesized peptide.