Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human Vimentin around the phosphorylation site of serine 83 (Q-D-SP-V-D).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P08670
Applications:
WB: 1:500~1:1000 ELISA: 1:10000
Other Names:
VIME, vimentin
Specificity Sensitivity:
Vimentin (Ab-83) antibody detects endogenous levels of total Vimentin protein.
References:
Ferrari S., Mol. Cell. Biol. 6:3614-3620(1986).
Hartmann T.B., Br. J. Dermatol. 150:252-258(2004).
Ota T., Nat. Genet. 36:40-45(2004).
Western blot analysis of extracts from 293 cells, treated with EGF (200ng/ml, 30mins), using Vimentin (Ab-83) antibody.