• Catalog Number:

  E1A1224B-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human PI3-kinase p85-α/γ around the phosphorylation site of tyrosine 468/199 (R-L-YP-E-E).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P27986

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000

• Other Names:

  GRB1; P85A; phosphatidylinositol 3-kinase regulatory alpha subunit; PI3-kinase p85-alpha; PI3K; PI3K p85-alpha; ptdIns-3-kinase p85-alpha

• Specificity Sensitivity:

  PI3-kinase p85-α/γ (Ab-467/199) antibody detects endogenous levels of total PI3-kinase p85-α/γ protein.

• References:

  Skolnik E.Y., Cell 65:83-90(1991). Antonetti D.A., Mol. Cell. Biol. 16:2195-2203(1996). Udelhoven M., Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases.

Peptide - +

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using PI3-kinase p85-α/γ (Ab-467/199) antibody. Western blot analysis of extracts from COS7 cells, treated with H2O2 (100μM, 30mins), using PI3-kinase p85-α/γ (Ab-467/199) antibody.

Peptide - + Immunofluorescence analysis of HeLa cells, using PI3-kinase p85-α/γ (Ab-467/199) antibody.