Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human S1P Receptor EDG1 around the phosphorylation site of threonine 236 (R-L-TP-F-R).
Purification:
The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse
SwissProtNo:
P21453
Applications:
WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000
Other Names:
EDG1, Probable G protein-coupled receptor EDG-1
Specificity Sensitivity:
S1P Receptor EDG1 (Ab-236) antibody detects endogenous levels of total S1P Receptor EDG1 protein.
References:
Hla T., J. Biol. Chem. 265:9308-9313(1990).
An S., FEBS Lett. 417:279-282(1997).
Parrill A.L., J. Biol. Chem. 275:39379-39384(2000).
Peptide - +
Western blot analysis of extracts from HT-29 cells, LOVO cells and A549 cells, using S1P Receptor EDG1 (Ab-236) antibody.
Immunofluorescence analysis of A549 cells, using S1P Receptor EDG1 (Ab-236) antibody.