Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human POU2F1/OCT1 around the phosphorylation site of serine 385 (R-T-SP-I-E).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse
SwissProtNo:
P14859
Applications:
WB: 1:500~1:1000 ELISA: 1:5000
Other Names:
NF-A1, OCT1, OTF-1, Oct-1, Octamer-binding transcription factor 1, PO21, PO2F1, POU domain, class 2, transcription factor 1, POU2F1
Specificity Sensitivity:
POU2F1/OCT1 (Ab-385) antibody detects endogenous levels of total POU2F1/OCT1 protein.
References:
Luchina N.N., Immunol. Lett. 85:237-241(2003).
Gregory S.G., Nature 441:315-321(2006).
Prefontaine G.G., J. Biol. Chem. 274:26713-26719(1999).
Western blot analysis of extracts from HepG2 cells (lane 1) and COLO205 cells (lane 2), using POU2F1/OCT1 (Ab-385) antibody.