Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human IL-10Rα around the phosphorylation site of tyrosine 496 (K-G-YP-L-K).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
IL-10Rα (Ab-496) antibody detects endogenous levels of total IL-10Rα protein.
References:
Liu Y., J. Immunol. 152:1821-1829(1994).
Zhang Z., Protein Sci. 13:2819-2824(2004).
Josephson K., Immunity 15:35-46(2001).
Peptide - +
Western blot analysis of extracts from HepG2 cells, treated with Na2VO3 (0.3nM, 40mins), using IL-10Rα (Ab-496) antibody.
Immunofluorescence analysis of HepG2 cells, using IL-10Rα (Ab-496) antibody.