Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human AurB around the phosphorylation site of threonine 232 (R-K-TP-M-C).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
Q96GD4
Applications:
WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000
Other Names:
AIK2, AIM-1, AIM1, ARK2, AURKB, Aurora- and Ipl1-like midbody-associated protein 1, Aurora-B, Aurora-related kinase 2, Aurora/IPL1-related kinase 2, EC 2.7.11.1, STK-1, STK12, Serine/threonine protein kinase 12
Specificity Sensitivity:
AurB (Ab-232) antibody detects endogenous levels of total AurB protein.
References:
Shindo M., Biochem. Biophys. Res. Commun. 244:285-292(1998).
Tatsuka M., Cancer Res. 58:4811-4816(1998).
Kimura M., Cytogenet. Cell Genet. 82:147-152(1998).
Peptide - +
Western blot analysis of extracts from COS7 Nocodazole cells, treated with Nocodazole (1ug/ml, 16hours), using AurB (Ab-232) antibody.
Immunofluorescence analysis of HeLa cells, using AurB (Ab-232) antibody.