• Catalog Number:

  E1A1004B-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human Smad3 around the phosphorylation site of serine 204(A-G-SP-P-N).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P84022

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000

• Other Names:

  JV15-2, MAD-3, MADH3, Mad3, Mothers against DPP homolog 3, Mothers against decapentaplegic homolog 3, SMAD 3, Smad 3

• Specificity Sensitivity:

  Smad3 (Ab-204) antibody detects endogenous levels of total Smad3 protein.

• References:

  Zhang Y., Nature 383:168-172(1996). Riggins G.J., Nat. Genet. 13:347-349(1996). Arai T., Cancer Lett. 122:157-163(1998).

Peptide - +

Immunohistochemistry analysis of paraffin-embedded human colon colon tissue using Smad3 (Ab-204) antibody. Western blot analysis of extracts from HUVEC cells, using Smad3 (Ab-204) antibody.