• Catalog Number:

  E1A0998B-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human Smad2 around the phosphorylation site of serine 250 (E-L-SP-P-T).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q15796

• Applications:

  WB: 1:500~1:1000 ELISA: 1:1000

• Other Names:

  JV18-1; Mad-related protein 2; MADH2; MADR2; Mothers against decapentaplegic homolog 2; Mothers against DPP homolog 2; Smad 2

• Specificity Sensitivity:

  Smad2 (Ab-250) antibody detects endogenous levels of total Smad2 protein.

• References:

  Riggins G.J., Nat. Genet. 13:347-349(1996). Zhang Y., Nature 383:168-172(1996). Eppert K., Cell 86:543-552(1996).

Western blot analysis of extracts from COLO cells, treated with serum (20%, 15mins), using Smad2 (Ab-250) antibody.