• Catalog Number:

  E1A0029B-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from humanSmad1 around the phosphorylation site of serine 465 (I-S-S-V-SP).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q15797

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000

• Other Names:

  BSP-1; BSP1; Dwarfin-A; Dwf-A; JV4-1; Mad-related protein 1; Mad1; MADH1; MADR1; Mothers against decapentaplegic homolog 1; Mothers against DPP homolog 1; Mothers-against-DPP-related-1; Smad 1; SMAD 1; Transforming growth factor- beta signaling protein-1

• Specificity Sensitivity:

  Smad1 (Ab-465) antibody detects endogenous levels of total Smad1 protein.

• References:

  Riggins G.J., Nat. Genet. 13:347-349(1996). Liu F., Nature 381:620-623(1996). Hoodless P.A., Cell 85:489-500(1996).

Peptide - +

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Smad1 (Ab-465) antibody. Western blot analysis of extracts from COS7 cells, treated with EGF (200ng/ml, 15mins), using Smad1 (Ab-465) antibody.