Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human SHP-2 around the phosphorylation site of tyrosine542 (H-E-YP-T-N).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
Q06124
Applications:
WB: 1:500~1000 IHC: 1:50~100
ELISA: 1:40000
Specificity Sensitivity:
SHP-2 (Ab-542) antibody detects endogenous levels of total SHP-2 protein.
References:
Minako Yamaoka-Tojo, Arterioscler. Thromb. Vasc. Biol., Sep 2006; 26: 1991 - 1997.
Liang Liang, Endocrinology, Sep 2000; 141: 3328.
Tony Avril, J. Immunol., Dec 2004; 173: 6841 - 6849.
Laura A. Maile, Mol. Biol. Cell, Sep 2003; 14: 3519.
Peptide - +
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using SHP-2 (Ab-542) antibody (#B0027).
Western blot analysis of extracts from A431 cells, using SHP-2 (Ab-542) antibody (#B0027, Line 1 and 2).