• Catalog Number:

  E1A0021B-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human NF-κB p105/p50 around the phosphorylation site of serine927 (C-D-SP-G-V).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P19838

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000

• Other Names:

  DNA-binding factor KBF1, EBP-1, NF-kappa-B1 p84/NF-kappa-B1 p98, NFKB1, NFkB-p50, Nuclear factor NF-kappa-B p105 subunit, nuclear factor NF-kappa-B p50 subunit

• Specificity Sensitivity:

  NF-κB p105/p50 (Ab-927) antibody detects endogenous levels of total NF-κB p105/p50 protein.

• References:

  John J. Haddad, J. Pharmacol. Exp. Ther., Feb 2002; 300: 567. Sabine Kirchhoff, Eur. J. Biochem., Apr 1999; 261: 546. Gerald Thiel, Eur. J. Biochem., Jul 2004; 271: 2855 – 2862.

Peptide - +

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NF-κB p105/p50 (Ab-927) antibody. Western blot analysis of extracts from Hela cells treated with TNF-α (20ng/ml, 5min), using NF-κB p105/p50 (Ab-927) antibody.

Peptide - + Immunofluorescence analysis of HeLa cells, using NF-κB p105/p50 (Ab-927) antibody.