• Catalog Number:

  E1A0015B-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human IκB-ε around the phosphorylation site of serine 22 (I-E-SP-L-R).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  O00221

• Applications:

  WB: 1:500~1000 IHC: 1:50~100 IF: 1:100~1:500 ELISA: 1:40000

• Alter Name:

  I-kappa-B-epsilon, IKBE, IkappaBepsilon, NF-kappa-BIE, NF-kappaB inhibitor epsilon, NFKBIE

• Specificity Sensitivity:

  IκB-ε (Ab-22) antibody detects endogenous levels of total IκB-ε protein.

• References:

  David C. Hay Mol, Cell. Biol., May 2001; 21: 3482 - 3490. Fumitaka Fujita Mol. Cell. Biol., Nov 2003; 23: 7780 - 7793. Dan Tapalaga J. Histochem. Cytochem., Dec 2002; 50: 1599.

Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IκB-ε (Ab-22) antibody (#B0015).

Western blot analysis of extracts from Hela cells treated with TNF-α (20ng/ml 5min), using IκB-ε (Ab-22) antibody (#B0015, Line 1 and 2).

Peptide - + Immunofluorescence analysis of HeLa cells, using IκB-ε (Ab-22) antibody (#B0015).