Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human p130 Cas around the phosphorylation site of tyrosine 410 (G-V-T-A-V).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P56945
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100
ELISA: 1:5000
Alter Name:
BCAR1,Breast cancer anti-estrogen resistance 1,CRK-ASSOCIATED SUBSTRATE, CRKAS
Specificity Sensitivity:
p130 Cas (Ab-410) antibody detects endogenous levels of total p130 Cas protein.
References:
Katarzyna Modzelewska, J. Biol. Chem., Dec 2006; 281: 37527 - 37535.
Kevin Ogden, Am J Physiol Heart Circ Physiol, Dec 2006; 291: H2857 - H2863.
Sangkil Nam, Cancer Res., Oct 2005; 65: 9185 - 9189.
Norikazu Yamana, Mol. Cell. Biol., Sep 2006; 26: 6844 - 6858.
Peptide - +
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p130 Cas (Ab-410) antibody (#B0077).
Western blot analysis of extracts from NIH/3T3 cells, using p130 Cas (Ab-410) antibody (#B0077).