Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human HSL around the phosphorylation site of serine 554 (S-V-SP-E-A).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
Q05469
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000
Other Names:
EC 3.1.1.79, Hormone sensitive lipase, LIPE, LIPS
Specificity Sensitivity:
HSL (Ab-554) antibody detects endogenous levels of total HSL protein.
References:
Matthew J. Watt, Am J Physiol Endocrinol Metab, Mar 2006; 290: E500 - E508.
Maya S. Gowri, Am J Physiol Endocrinol Metab, Sep 2000; 279: E593 - E600.
Matthew J. Watt, Am J Physiol Endocrinol Metab, Jul 2004; 287: E120 - E127.
Peptide - +
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using HSL (Ab-554) antibody.
Western blot analysis of extracts from HeLa cells, treated with Calyculin A (100nM, 30mins), using HSL (Ab-554) antibody.