• Catalog Number:

  E1A0068B-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human Cyclin B1 around the phosphorylation site of serine 126 (T-A-S-P-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P14635

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000

• Alter Name:

  CCN-2, CCNB, CCNB1, CCNB1-RS1, CYCB, CYCB1, G2/mitotic-specific cyclin B1

• Specificity Sensitivity:

  Cyclin B1 (Ab-126) antibody detects endogenous levels of total Cyclin B1 protein.

• References:

  Jing Yang, J. Biol. Chem., Jan 2001; 276: 3604 - 3609. Susan Walsh, Mol. Cancer Res., Feb 2003; 1: 280. Florian Bassermann, J. Biol. Chem., Mar 2007; 10.1074. Jianke Li, PNAS, Jan 1997; 94: 502.

Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Cyclin B1 (Ab-126) antibody (#BOO68).

Western blot analysis of extracts from HeLa cells, treated with serum (10%, 15min), using Cyclin B1 (Ab-126) antibody (#BOO68, Line 1 and 2).

Forskolin + - Immunofluorescence analysis of HeLa cells, treated with Forskolin (40nM, 30mins), using Cyclin B1 (Ab-126) antibody (#BOO68).