• Catalog Number:

  E1A0064B-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human COT around the phosphorylation site of threonine 290 (R-G-TP-E-I).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P41279

• Applications:

  WB: 1:300~1000 IHC: 1:50~100 IF: 1:100~1:500 ELISA: 1:40000

• Alter Name:

  C-COT, COT proto-oncogene serine/threonine-protein kinase, Cancer Osaka thyroid oncogene, EC 2.7.11.25, M3K8, MAP3K8, Mitogen-activated protein kinase kinase kinase 8, TPL-2, TPL2, Tpl2, Tumor progression locus 2, kinase Cot

• Specificity Sensitivity:

  COT (Ab-290) antibody detects endogenous levels of total COT protein.

• References:

  Lawrence P. Kane, Mol. Cell. Biol., Aug 2002; 22: 5962 - 5974. Brenda S. Luciano, J. Biol. Chem., Dec 2004; 279: 52117 - 52123. Matilde Caivano, J. Biol. Chem., Dec 2003; 278: 52124 - 52130. Kenji Sugimoto, J. Clin. Invest., Sep 2004; 114: 857 - 866.

Peptide - + Immunohistochemical analysis of paraffin-embedded human brain tissue using COT (Ab-290) antibody (#B0064).

Western blot analysis of extracts from 293 cells treated with LPS (100ng/ml, 30min), using COT (Ab-290) antibody (#B0064, Line 1 and 2).

Peptide - + Immunofluorescence analysis of HuvEc cells, using COT (Ab-290) antibody (#B0064).