Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human LIMK1/2 around the phosphorylation site of threonine 508/505 (R-Y-TP-V-V).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
LIMK1/2 (Ab-508/505) antibody detects endogenous levels of total LIMK1/2 protein.
References:
C. CHANDRA KUMAR, Ann. N.Y. Acad. Sci., Jan 1999; 886: 122.
Daniel R. Croft, Molecular and Cellular Biology, June 2006, p. 4612-4627, Vol. 26.
Daniel R. Croft,JCB, Volume 168, Number 2, 245-255.
Xinyu Chen, JCB, Volume 172, Number 5, 671-678.
Peptide - +
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using LIMK1/2 (Ab-508/505) antibody (#B0047).
Western blot analysis of extracts from cos7 cells, using LIMK1/2 (Ab-508/505) antibody (#B0047, Line 1 and 2).