• Catalog Number:

  E1A0042B-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human Akt around the phosphorylation site of threonine 308 (M-K-TP-F-C).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P31749

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

• Other Names:

  AKT, AKT1 kinase, C-AKT, EC 2.7.11.1, PKB, PKB-alpha, Protein kinase B, RAC, RAC-PK-alpha, RAC-alpha serine/threonine kinase, kinase Akt1

• Specificity Sensitivity:

  Akt (Ab-308) antibody detects endogenous levels of total Akt protein.

• References:

  Jones P.F., Proc. Natl. Acad. Sci. U.S.A. 88:4171-4175(1991). Matsubara A., Diabetologia 44:910-913(2001). Coffer P.J., Eur. J. Biochem. 201:475-481(1991).

Peptide - +

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Akt (Ab-308) antibody. Western blot analysis of extracts from 293 cells, treated with EGF (200ng/ml, 30mins), using Akt (Ab-308) antibody.