Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human Trk B around the phosphorylation site of tyrosine 515 (P-Q-YP-F-G).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Trk B (Ab-515) antibody detects endogenous levels of total Trk B protein.
References:
Tina Garofalo, J. Biol. Chem., Dec 1998; 273: 35153.
VI Shifrin and BG Neel, J. Biol. Chem., Dec 1993; 268: 25376 - 25384.
Jan Grimm, J. Cell Biol., Jul 2001; 154: 345.
Jose L. Tomsig and Terry T. Turner, J. Androl, May 2006; 27: 348 - 357.
Peptide - +
Immunohistochemical analysis of paraffin-embedded human brain tissue using Trk B (Ab-515) antibody (#B0035).
Western blot analysis of extracts from 3T3 cells treated with PBS (60min), using Trk B (Ab-515) antibody (#B0035, Line 1 and 2).