• Catalog Number:

  E1A0001B-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human 14-3-3 ζ around the phosphorylation site of serine 58 (R-S-SP-W-R).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P63104

• Applications:

  WB: 1:500~1000 IHC: 1:50~100 IP: Various Dilution IF: 1:100~1:500 ELISA: 1:40000

• Alter Name:

  14-3-3 protein zeta/delta, 1433Z, 143Z, FAS, Factor activating exoenzyme S, KCIP-1, Mitochondrial import stimulation factor S1 subunit, Protein kinase C inhibitor protein-1, YWHAZ

• Specificity Sensitivity:

  14-3-3 ζ (Ab-58) antibody detects endogenous levels of total 14-3-3 ζ protein.

• References:

  Ying H. Shen Mol. Biol. Cell, Nov 2003; 14: 4721. David W. Powell, Mol. Cell. Biol., Aug 2003; 23: 5376 - 5387. Mackintosh. C, (2004) Biochem. J. 381, 329 - 42. Dougherty, M.K. and Morrison, D.K. (2004) J. Cell Sci. 117, 1875 - 84.

Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 14-3-3 ζ (Ab-58) antibody (#B0001).

Western blot analysis of extracts from 293 cells treated with Forskolin (40nM, 30min) using 14-3-3 ζ (Ab-58) antibody (#B0001, Line1 and 2).

PMA + - Immunofluorescence analysis of HeLa cells, treated with PMA (125ng/ml, 30mins), using 14-3-3 ζ (Ab-58) antibody (#B0001).