Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized peptide derived from Internal of human TOP1.
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human; Mouse; Rat
SwissProtNo:
P11387
Applications:
WB: 1:500~1:3000 ELISA: 1:40000
Other Names:
DNA topoisomerase 1; DNA topoisomerase I; TOP1; TOPI; topoisomerase (DNA) I; type I DNA topoisomerase
Specificity Sensitivity:
TOP1 antibody detects endogenous levels of total TOP1 protein.
References:
D'Arpa P., Proc. Natl. Acad. Sci. U.S.A. 85:2543-2547(1988).
Kunze N., J. Biol. Chem. 266:9610-9616(1991).
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Western blot analysis of extracts from Jurkat/293 cells, using TOP1 antibody.