Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized peptide derived from internal of human POLR1E.
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human (Identities = 100%, Positives = 100%)
SwissProtNo:
Q9GZS1
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
Other Names:
DNA-directed RNA polymerase I RPA49; DNA-directed RNA polymerase I subunit E; PAF53; polymerase (RNA) I polypeptide E, 53kDa; PRAF1; RNA polymerase I A49; RNA polymerase I-associated factor 1; RNA polymerase I-associated factor 53; RPA49
Specificity Sensitivity:
POLR1E antibody detects endogenous levels of total POLR1E protein.
References:
Ota T., Nat. Genet. 36:40-45(2004).
Ebert L., Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Humphray S.J., Nature 429:369-374(2004).
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Peptide - +
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using POLR1E antibody.
Western blot analysis of extracts from MCF-7 cells, using POLR1E antibody.