Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized peptide derived from internal of human DNL1.
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human
SwissProtNo:
P18858
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
Other Names:
DNA ligase 1, EC 6.5.1.1, DNA ligase I, Polydeoxyribonucleotide synthase [ATP] 1, LIG1
Specificity Sensitivity:
DNL1 antibody detects endogenous levels of total DNL1 protein.
References:
Barnes D.E., Proc. Natl. Acad. Sci. U.S.A. 87:6679-6683(1990).
Petrini J.H.J., Proc. Natl. Acad. Sci. U.S.A. 88:7615-7619(1991).
Beausoleil S.A., Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004).
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Peptide - +
Immunohistochemistry analysis of paraffin-embedded human brain tissue using DNL1 antibody.
Western blot analysis of extracts from HT-29 cells, using DNL1 antibody.