Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized peptide derived from internal of human DFF-45.
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
O00273
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
Other Names:
DNA fragmentation factor subunit alpha, DNA fragmentation factor 45 kDa subunit, DFF-45, Inhibitor of CAD, ICAD, DFFA, DFF1, DFF45, H13
Specificity Sensitivity:
DFF-45 antibody detects endogenous levels of total DFF-45 protein.
References:
Liu X.,, Cell 89:175-184(1997).
Gu J.J., J. Biol. Chem. 274:20759-20762(1999).
Oh J.J., Nucleic Acids Res. 27:4008-4017(1999).
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Peptide - +
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using DFF-45 antibody.
Western blot analysis of extracts from 293 cells, Jurkat cells and HeLa cells, using DFF-45 antibody.