Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized peptide derived from internal of human 14-3-3 β.
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P31946
Applications:
WB: 1:500~1:1000 ELISA: 1:10000
Other Names:
14-3-3 protein beta/alpha, 1433B, 143B, KCIP-1, Protein 1054, Protein kinase C inhibitor protein-1, YWHAB
Specificity Sensitivity:
14-3-3 β antibody detects endogenous levels of total 14-3-3 β protein.
References:
Leffers H., J. Mol. Biol. 231:982-998(1993).
Deloukas P., Nature 414:865-871(2001).
Gevaert K., Nat. Biotechnol. 21:566-569(2003).
Chi A., J. Proteome Res. 5:3135-3144(2006).
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Western blot analysis of extracts from HepG2 cells and Jurkat cells, using 14-3-3 β antibody.