Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized peptide derived from C-terminal of human GIT1.
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human (Identities = 100%, Positives = 100%);Mouse (Identities = 100%, Positives = 100%);
Rat (Identities = 100%, Positives = 100%)
SwissProtNo:
Q9Y2X7
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
Other Names:
ARF GTPase-activating protein GIT1, G protein-coupled receptor kinase- interactor 1, similar to G protein-coupled receptor kinase-interactor 1; G protein-coupled receptor kinase-associated ADP ribosylation factor GTPase-activating protein
Specificity Sensitivity:
GIT1 antibody detects endogenous levels of total GIT1 protein.
References:
Premont R.T., J. Biol. Chem. 275:22373-22380(2000).
The MGC Project Team, Genome Res. 14:2121-2127(2004).
Premont R.T., Proc. Natl. Acad. Sci. U.S.A. 95:14082-14087(1998).
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Peptide - +
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using GIT1 antibody.
Western blot analysis of extracts from HeLa cells, Jurkat cells and HepG2 cells, using GIT1 antibody.