Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized peptide derived from internal of human ASC.
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
Q9ULZ3
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000
Other Names:
Apoptosis-associated speck-like protein containing a CARD, hASC, PYD and CARD domain-containing protein, Target of methylation-induced silencing 1, Caspase recruitment domain-containing protein 5, PYCARD, ASC, CARD5, TMS1
Specificity Sensitivity:
ASC antibody detects endogenous levels of total ASC protein.
References:
Masumoto J.,, J. Biol. Chem. 274:33835-33838(1999).
Conway K.E., Cancer Res. 60:6236-6242(2000).
McConnell B.B., Cancer Res. 60:6243-6247(2000).
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Peptide - +
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using ASC antibody.
Western blot analysis of extracts from RAW264.7 cells, using ASC antibody.