Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized peptide derived from N-terminal of human DBF4.
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse
SwissProtNo:
Q9UBU7
Applications:
WB: 1:500~1:1000 ELISA: 1:10000
Other Names:
Protein DBF4 homolog A, Activator of S phase kinase, DBF4-type zinc finger-containing protein 1, Chiffon homolog A, DBF4, ASK, DBF4A, ZDBF1
Specificity Sensitivity:
DBF4 antibody detects endogenous levels of total DBF4 protein.
References:
Kumagai H., Mol. Cell. Biol. 19:5083-5095(1999).
Jiang W., EMBO J. 18:5703-5713(1999).
Ota T., Nat. Genet. 36:40-45(2004).
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Western blot analysis of extracts from NIH-3T3 cells treated with H2O2 (100uM, 30mins) and COS-7 cells treated with PMA (125mg/ml, 30mins), using DBF4 antibody.