• Catalog Number:

  E1A10558C-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized peptide derived from N-terminal of human Actin α-2/3.

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P35609/Q08043

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000

• Other Names:

  Alpha-actinin-2, Alpha-actinin skeletal muscle isoform 2, F-actin cross-linking protein, ACTN2

• Specificity Sensitivity:

  Actin α-2/3 antibody detects endogenous levels of total Actin α-2/3 protein.

• References:

  Beggs A.H., J. Biol. Chem. 267:9281-9288(1992). Tiso N., Biochem. Biophys. Res. Commun. 265:256-259(1999). Faulkner G., J. Cell Biol. 146:465-475(1999). ------------------------------------------------------------------------------------------------------------------------------------------

Peptide - +

Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue using Actin α-2/3 antibody. Western blot analysis of extracts from HeLa cells, HepG2 cells and HUVEC cells, using Actin α-2/3 antibody.

Peptide - + Immunofluorescence analysis of HeLa cells, using Actin α-2/3 antibody.