Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized peptide derived from N-terminal of human AKAP5.
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human
SwissProtNo:
P24588
Applications:
WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:10000
Other Names:
A-kinase anchor protein 5, A-kinase anchor protein 79 kDa, AKAP 79, cAMP-dependent protein kinase regulatory subunit II high affinity-binding protein, H21, AKAP5, AKAP79
Specificity Sensitivity:
AKAP5 antibody detects endogenous levels of total AKAP5 protein.
References:
Carr D.W., J. Biol. Chem. 267:16816-16823(1992).
Hirsch A.H., J. Biol. Chem. 267:2131-2134(1992).
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Peptide - +
Western blot analysis of extracts from HepG2 cells, HeLa cells and COLO205 cells, using AKAP5 antibody.
Immunofluorescence analysis of HeLa cells, using AKAP5 antibody.