Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized peptide derived from internal of human S6K.
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P23443
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000
Other Names:
EC 2.7.11.1, KS6B1, P70-S6K, RPS6KB1, Ribosomal protein S6 kinase, Ribosomal protein S6 kinase, 70kDa, polypeptide 1, S6K, kinase p70S6K, p70-S6K
Specificity Sensitivity:
S6K antibody detects endogenous levels of total S6K protein.
References:
Grove J., Mol. Cell. Biol. 11:5541-5550(1991).
Gout I., J. Biol. Chem. 273:30061-30064(1998).
Olsen J.V., Cell 127:635-648(2006).
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Peptide - +
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using S6K antibody.
Western blot analysis of extracts from COLO205 cells and Jurkat cells, using S6K antibody.