• Catalog Number:

  E1A10345C-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized peptide derived from N-terminal of human TOP2A.

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human

• SwissProtNo:

  P11388

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000

• Other Names:

  DNA topoisomerase 2-alpha, EC 5.99.1.3, DNA topoisomerase II, alpha isozyme, TOP2A, TOP2

• Specificity Sensitivity:

  TOP2A antibody detects endogenous levels of total TOP2A protein.

• References:

  Tsai-Pflugfelder M., Proc. Natl. Acad. Sci. U.S.A. 85:7177-7181(1988). Wasserman R.A., Cancer Res. 53:3591-3596(1993). Lang A.J., Gene 221:255-266(1998). ------------------------------------------------------------------------------------------------------------------------------------------

Peptide - +

Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue using TOP2A antibody. Western blot analysis of extracts from HT-29 cells, using TOP2A antibody.

Peptide - + Immunofluorescence analysis of A549 cells, using TOP2A antibody.