Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized peptide derived from internal of human CDC2.
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P06493
Applications:
WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:5000
Other Names:
CDC28, CDC2A, CDK1, Cell division control protein 2 homolog, Cyclin-dependent kinase 1, EC 2.7.11.22, EC 2.7.11.23, MPF, kinase Cdc2, p34 protein kinase
Specificity Sensitivity:
CDC2 antibody detects endogenous levels of total CDC2 protein.
References:
Lee M.G., Nature 327:31-35(1987).
Ohta T., Cancer Res. 58:1095-1098(1998).
Draetta G., Cell 54:17-26(1988).
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Peptide - +
Western blot analysis of extracts from Jurkat cells, COLO205 cells and HUVEC, using CDC2 antibody.
Immunofluorescence analysis of HUVEC cells, using CDC2 antibody.