• Catalog Number:

  E1A10257C-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized peptide derived from internal of human EGFR.

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human (Identities = 100%, Positives = 100%)Mouse (Identities = 100%, Positives = 100%) Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P00533

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

• Other Names:

  epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian); Epidermal growth factor receptor precursor; ERBB1; kinase EGFR; Receptor protein-tyrosine kinase ErbB-1

• Specificity Sensitivity:

  EGFR antibody detects endogenous levels of total EGFR protein.

• References:

  Seeburg P.H., Nature 309:418-425(1984). Henzel W.J., Protein Sci. 13:2819-2824(2004). Kohda D., J. Biochem. 127:65-72(2000). ------------------------------------------------------------------------------------------------------------------------------------------

Western blot analysis of extracts from A549, HeLa and HepG2 cells, using EGFR antibody.