Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized peptide derived from Internal of human vimentin.
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P08670
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
Other Names:
VIME, vimentin
Specificity Sensitivity:
Vimentin antibody detects endogenous levels of total vimentin protein.
References:
WB Isaacs, J. Biol. Chem., Oct 1989; 264: 17953 - 17960.
Patric Turowski, Mol. Biol. Cell, Jun 1999; 10: 1997.
Melanie L. Styers, J. Cell Sci., Sep 2006; 119: 3643 - 3654.
Qing-Fen Li, J. Biol. Chem., Nov 2006; 281: 34716 - 34724.
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Peptide - +
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Vimentin antibody.
Western blot analysis of extracts from HepG2 cells, treated with Adriamycin (0.5uM, 5hours), using Vimentin antibody.