Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized peptide derived from Internal of human topoisomerase II β.
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse
SwissProtNo:
Q02880
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
Other Names:
DNA topoisomerase II, beta isozyme, EC 5.99.1.3, TP2B, TOP2B
Specificity Sensitivity:
Topoisomerase II β antibody detects endogenous levels of total topoisomerase II β protein.
References:
René M. Linka, Nucleic Acids Res., May 2007; 10.1093.
John R. Jenkins, Nucleic Acids Res., Nov 1992; 20: 5587 - 5592.
Kathryn L. Gilroy, Nucleic Acids Res., Mar 2006; 34: 1597 - 1607.
Sophie Dereuddre, Cancer Res., Oct 1997; 57: 4301 - 4308.
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Peptide - +
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using Topoisomerase II β antibody.
Western blot analysis of extracts from Jurkat cells, using Topoisomerase II β antibody.