• Catalog Number:

  E1A0365L-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized peptide derived from human PARP.

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P09874

• Applications:

  WB: 1:500~1:1000 ELISA: 1:20000

• Other Names:

  Poly [ADP-ribose] polymerase 1, EC 2.4.2.30, PARP-1, ADPRT, NAD(+) ADP-ribosyltransferase 1, Poly[ADP-ribose] synthetase 1, PARP1, ADPRT, PPOL

• Specificity Sensitivity:

  PARP (Cleaved-Asp214) antibody detects endogenous levels of fragment of activated PARP resulting from cleavage adjacent to Asp214. This antibody is only showing cleaved form. Observed band size : 26kd.

• References:

  Auer B., DNA 8:575-580(1989). Uchida K., Biochem. Biophys. Res. Commun. 148:617-622(1987). Kurosaki T., J. Biol. Chem. 262:15990-15997(1987).

Western blot analysis of extracts from A549 cells, treated with etoposide (25uM, 24hours), using PARP (Cleaved-Asp214) antibody.