Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized peptide derived from Internal of human NEDD8.
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
SwissProtNo:
Q15843
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000
Other Names:
NED8, NEDD-8, Ubiquitin-like protein NEDD8
Specificity Sensitivity:
NEDD8 antibody detects endogenous levels of total NEDD8 protein.
References:
Margaret A. Read, Mol. Cell. Biol., Apr 2000; 20: 2326 - 2333.
Kenneth Wu, J. Biol. Chem., Aug 2003; 278: 28882 - 28891.
Katsumi Kito, J. Biol. Chem., Jun 2001; 276: 20603 - 20609.
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Peptide - +
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using NEDD8 antibody.
Western blot analysis of extracts from Jurkat cells, using NEDD8 antibody.