Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized peptide derived from human PCNA.
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P12004
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100
ELISA: 1:5000
Alter Name:
cyclin, proliferating cell nuclear antigen
Specificity Sensitivity:
PCNA antibody detects endogenous levels of total PCNA protein.
References:
Anje Sporbert, Nucleic Acids Res., Jun 2005; 33: 3521 - 3528.
Sean C. Gehen, Am J Physiol Lung Cell Mol Physiol, Mar 2007; 292: L716 - L724.
Basavaraj Bagewadi, J. Virol., Nov 2004; 78: 11890 - 11903.
T Iida, I Suetake, Genes Cells, Oct 2002; 7: 997 - 1007.
Peptide - +
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PCNA antibody (#C0298).
Western blot analysis of extracts from HepG2 cells, using PCNA antibody (#C0298).