Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized peptide derived from N-terminal of human p300.
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human
SwissProtNo:
Q09472
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
Other Names:
E1A-associated protein p300, EP300, P300 transcription coactivator homolog
Specificity Sensitivity:
p300 antibody detects endogenous levels of total p300 protein.
References:
Wuchao Yuan, J. Biol. Chem., Apr 1996; 271: 9009.
E. Tory Manning, Mol. Cell. Biol., Jun 2001; 21: 3876 - 3887.
Jiwen Li, Mol. Cell. Biol., Mar 2000; 20: 2031 - 2042.
Gopalswamy Jayaraman, J. Biol. Chem., Jun 1999; 274: 17342.
Peptide - +
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using p300 antibody.
Western blot analysis of extracts from MDA-MB-435 cells, using p300 antibody.