Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized peptide derived from human osteopontin .
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P10451
Applications:
WB: 1:500~1:1000 ELISA: 1:20000
Alter Name:
Bone sialoprotein 1, Nephropontin, OPN, OSTP, SPP-1, SPP1, Secreted phosphoprotein 1, Urinary stone protein, Uropontin
Specificity Sensitivity:
Osteopontin antibody detects endogenous levels of total osteopontin protein.
References:
George R. Beck, J. Biol. Chem., Oct 2003; 278: 41921 - 41929.
KELLY L. HUDKINS, J. Am. Soc. Nephrol., Mar 1999; 10: 444.
Lígia R. Rodrigues, Cancer Epidemiol. Biomarkers Prev., Jun 2007; 16: 1087 - 1097.
Yu-Hua Hsieh, Cancer Res., Jul 2006; 66: 7119 - 7127.
Western blot analysis of extracts from LOVO cells, using osteopontin antibody (#C0284).