Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized peptide derived from human MMP-9.
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human
SwissProtNo:
P14780
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100
ELISA: 1:5000
Alter Name:
92 kDa gelatinase, 92 kDa type IV collagenase precursor, CLG4B, GELB, Gelatinase B, MM09
Specificity Sensitivity:
MMP-9 antibody detects endogenous levels of total MMP-9 protein.
References:
Martin Illemann, Mol. Cancer Res., May 2006; 4: 293 - 302. Noemi Ramos-DeSimone, J. Biol. Chem., May 1999; 274: 13066. Vandana Iyer, J. Cell Sci., Mar 2005; 118: 1185 - 1195. Jon R. Backstrom, J. Neurosci., Dec 1996; 16: 7910.
Peptide - +
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MMP-9 antibody (#C0275).
Western blot analysis of extracts from HepG2 cells, using MMP-9 antibody (#C0275).