Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized peptide derived from human MART-1.
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human
SwissProtNo:
Q16655
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100
ELISA: 1:40000
Alter Name:
MART-1, MART1, melan-A, MELAN A, MELANOMA ANTIGEN RECOGNIZED BY T CELLS 1
Specificity Sensitivity:
MART-1 antibody detects endogenous levels of total MART-1 protein.
References:
Toshihiko Hoashi, J. Biol. Chem., Apr 2005; 280: 14006 - 14016.
Maria P. Bettinotti, J. Immunol., Jul 1998; 161: 877.
P. J. Frost, J. Immunol., Mar 2001; 166: 3564.
Flora Wang, Clin. Cancer Res., Oct 1999; 5: 2756.
Peptide - +
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MART-1 antibody (#C0257).
Western blot analysis of extracts from NIH/3T3 cells, using MART-1 antibody (#C0257).