Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized peptide derived from human Keratin 10.
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Rat
SwissProtNo:
P13645
Applications:
WB: 1:500~1:1000 IF: 1:100~1:500
ELISA: 1:5000
Alter Name:
Keratin type I cytoskeletal 10, Cytokeratin-10, CK-10, K10
Specificity Sensitivity:
Keratin 10 antibody detects endogenous levels of total Keratin 10 protein.
References:
XM Zhou, J. Biol. Chem., Oct 1988; 263: 15584 - 15589.
Felix B. Müller, Hum. Mol. Genet., Apr 2006; 15: 1133 - 1141.
J Reichelt, J. Cell Sci., Sep 1997; 110: 2175.
BP Korge, PNAS, Feb 1992; 89: 910.
Peptide - +
Western blot analysis of extracts from HeLa cells, using Keratin 10 antibody (#C0249).
Immunofluorescence analysis of A549 cells, using Keratin 10 antibody (#C0249).