Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized peptide derived from human Keratin 8.
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P05787
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100
ELISA: 1:40000
Alter Name:
Keratin, type II cytoskeletal 8, Cytokeratin-8, CK-8, K8
Specificity Sensitivity:
Keratin 8 antibody detects endogenous levels of total Keratin 8 protein.
References:
Nam-On Ku, N. Engl. J. Med., May 2001; 344: 1580 - 1587.
Guo-Zhong Tao, J. Cell Sci., Nov 2003; 116: 4629.
Heather A.Long, J. Cell Sci., Dec 2006; 119: 5147 - 5159.
D. W. Owens, J. Cell Sci., Apr 2004; 117: 1989 - 1999.
Peptide - +
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Keratin 8 antibody (#C0248).
Western blot analysis of extracts from SKOV3 cells, using Keratin 8 antibody (#C0248).