• Catalog Number:

  E1A0227L-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized peptide derived from human Collagen IV α3.

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human

• SwissProtNo:

  Q01955

• Applications:

  WB: 1:500~1:1000 ELISA: 1:1000

• Other Names:

  Collagen alpha-3(IV) chain; Goodpasture antigen; Tumstatin; COL4A3; CO4A3

• Specificity Sensitivity:

  Collagen IV α3 (Cleaved-Leu1425) antibody detects endogenous levels of fragment of activated Collagen IV α3 resulting from cleavage adjacent to Leu1425.

• References:

  Mariyama M., J. Biol. Chem. 269:23013-23017(1994). Leinonen A., Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases. Heidet L., J. Am. Soc. Nephrol. 12:97-106(2001).

Western blot analysis of extracts from293 cells, treated with etoposide (25uM, 1hour), using Collagen IV α3 (Cleaved-Leu1425) antibody.