• Catalog Number:

  E1A0217C-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized peptide derived from human GRP78.

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P11021

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000

• Alter Name:

  BIP, GLUCOSE-REGULATED PROTEIN 78-KD, HEAT-SHOCK 70-KD PROTEIN 5, IMMUNOGLOBULIN HEAVY CHAIN-BINDING PROTEIN, heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)

• Specificity Sensitivity:

  GRP78 antibody detects endogenous levels of total GRP78 protein.

• References:

  Uma K. Misra, J. Biol. Chem., Oct 2002; 277: 42082 - 42087. CA Clairmont, J. Biol. Chem., Feb 1992; 267: 3983 - 3990. J Tooze, J. Cell Biol., Jul 1989; 109: 35. Jong K. Yun, PNAS, Dec 1997; 94: 13903.

Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GRP78 antibody (#C0217).

Western blot analysis of extracts from COS7 cells, using GRP78 antibody (#C0217).

Peptide - + Immunofluorescence analysis of COS7 cells, using GRP78 antibody (#C0217).