Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized peptide derived from human Collagen III α1.
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human
SwissProtNo:
P02461
Applications:
WB: 1:500~1:1000 ELISA: 1:10000
Other Names:
Collagen alpha-1(III) chain [Precursor], COL3A1
Specificity Sensitivity:
Collagen III α1 (Cleaved-Gly1221) antibody detects endogenous levels of fragment of activated Collagen III α1 resulting from cleavage adjacent to Gly1221.
References:
Ala-Kokko L., Biochem. J. 260:509-516(1989).
Valkkila M., Matrix Biol. 20:357-366(2001).
Hillier L.W., Nature 434:724-731(2005).
Western blot analysis of extracts from A549 cells, treated with etoposide (25uM, 24hours), using Collagen III α1 (Cleaved-Gly1221) antibody.