Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized peptide derived from human FLI1.
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse
SwissProtNo:
Q01543
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100
ELISA: 1:10000
Alter Name:
EWSR2, SIC-1, FLI1/EWS FUSION GENE, VIRAL INTEGRATION REGION FLI1, MOUSE HOMOLOG OF EWING SARCOMA BREAKPOINT REGION 2
Specificity Sensitivity:
FLI1 antibody detects endogenous levels of total FLI1 protein.
References:
Hana Raslova, J. Clin. Invest., Jul 2004; 114: 77 - 84.
Jeffrey A. Toretsky, Cancer Res., Jun 2006; 66: 5574 - 5581.
Masahide Kubo, Am. J. Pathol., Aug 2003; 163: 571.
Sashidhar S. Nakerakanti, J. Biol. Chem., Sep 2006; 281: 25259 - 25269.
Peptide - +
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using FLI1 antibody (#C0196).
Western blot analysis of extracts from NIH/3T3 cells, using FLI1 antibody (#C0196).